An Eadie-Hofstee plot has values of φ0​ on the ordinate and vy​... | Filo
An Eadie-Hofstee plot has values of φ0​ on the ordinate and vy​... | Filo
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Eadie-Hofstee Plot and Catalytic ConstantThere are different ... | Filo
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Question 2 Data shown in the table below were obtained for the hydrolysis of O-nitrophenyl-?-D- galactoside by E.coli ?-galactosidase. Construct i) Lineweaver-Burk, ii) Eadie-Hofstee and iii) Hanes plots to analyse the data and calculate KM and Vmax from each plot. [S] mM v0 (µmol/ml/min) 0.25 5 0.5 8.93 1.0 14.29 1.5 16.52 2.0 19.20 2.5 19.64 3 19.7 [12]
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