MTT Assay– A Simple tool for measuring Cell Viability #cell #assay #biotechnology
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MTT Assay– A Simple tool for measuring Cell Viability #cell #ass…
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Biologist🔬Ph.D on Instagram: "Bu videoda hücre canlılığının değerlendirilmesinde yaygın olarak kullanılan MTT testinin uygulanma süreci gösterilmektedir. MTT, metabolik olarak aktif hücrelerde mitokondriyal dehidrogenaz enzimleri tarafından indirgenerek çözünmeyen mor formazan kristallerine dönüşür. Oluşan formazan miktarı, hücrelerin metabolik aktivitesi ve dolaylı olarak canlılıkları ile doğru orantılıdır. Reaksiyon sonrasında kristaller uygun çözücü ile çözündürülerek absorbans ölçümü
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