You will isolate RNA from the two different types of tissue. You will make cDNA, label it fluorescently, and incubate each cDNA population with a separate microarray. You will isolate DNA from the two different types of tissue. You will label it fluorescently, and incubate both DNA populations with one microarray.

A DNA microarray is a glass slide with thousands of single-stranded DNA fragments fixed to it. Each fixed DNA fragment is from a particular gene. Therefore, a single microarray has DNA from thousands of genes. Biologists use DNA microarrays to figure out which genes are being transcribed by organisms in different situations.

Question: DNA Microarrays Drag each label to its proper place in the flow chart to summarize the steps involved in a DNA microarray analysis. examine fluorescence on microarray produce cDNA from mRNA rinse to remove unbound cDNA H add cDNA to microarray wells isolate mRNA from target cells 1 Rese

BIOTECHNOLOGY!!! 1. A DNA microarray is used to quantify mRNA levels. Why is it called DNA microarray then, instead of RNA microarray? 2. What is the advantage of DNA microarrays over other methods to study gene transcription? 3. In this particular experiment, what was the objective of the DNA microarray experiment? 4. In part 3 (isolate mRNA ...

Question: DNA Microarray technology can be used to (select all that apply) Determine which genes are translated into protein:s Determine what genes change expression as a consequence of an experimental perturbation Determine what genes are transcribed in a tissue Discover novel RNA transcripts not arrayed on the platform

When cDNAs are added to a DNA microarray, complementary sequences that anneal with oligonucleotides on the microarray must be identified by eye. Many thousands of different oligonucleotides are found on a single DNA microarray. Each spot on a DNA microarray contains thousands of copies of an oligonucleotide of approximately 20 bases.

A DNA microarray detects Multiple Choice uncommon forms of alleles, or mutants, associated with disease. an individual's complete genotype, including all the various mutations. chromosomal abnormalities, such as duplications or deletions. fetal cells in the mother's blood uncommon forms of alleles, or mutants, associated with disease. an individual's complete genotype, including all …

Question: Order the steps required to analyze gene expression from a particular cell type using a DNA microarray. First step Last step Answer Bank reverse transcribe mRNA to cDNA add cDNA to microarray and incubate wash away unbound cDNA label cDNA with a chemiluminescent molecule extract mRNA from cells

Consider the definition of a DNA Microarray: it's a collection of DNA sequences attached to a solid surface used for detecting mRNA or cDNA. Step 1 Introduction:

a DNA microarray (also called a DNA chip) can be used to____ A) detect RFLPs. B) generate mutations in a gene of interest. C) detect mutations that may indicate a risk of disease. D) determine protein translation levels. If you could explain the reasoning it would really help, thanks!