Sep 12, 2024 · The heterocylic diamidine DB2447 recognizes a single GC base pair in the minor groove of a mixed DNA sequence. The DNA is stabilized by a PU.1 protein bound to the major …

The chemical structure of DNA base-pairs A base pair (bp) is a fundamental unit of double-stranded nucleic acids consisting of two nucleobases bound to each other by hydrogen bonds. …

Denaturing gradient gel electrophoresis (DGGE) can be used to distinguish two DNA molecules that differ by as little as a single-base substitution. This method detects approximately 50% of …

Sep 18, 2024 · Here, we report the first X-ray crystal structures that demonstrate single G (guanine) recognition by a highly selective diamidine (DB2447) in a mixed DNA sequence. The …

Sep 20, 2024 · The study presents detailed structural information on the mechanism of single G recognition by D2447 and its various interactions in the DNA minor groove. Molecular …

The strong footprint at -GCTCG- for DB1242 is a unique GC-rich DNA recognition sequence with only one AT base pair, in contrast to the usual required four ATs with no GC base pair for the …

Sep 12, 2024 · The high-resolution NMR structure of the first heterocyclic, non-amide, organic cation that strongly and selectively recognizes mixed AT/GC bp (bp=base pair) sequences of …

Jun 19, 1999 · Use of p K a Differences To Enhance the Formation of Base Triplets Involving C−G and G−C Base Pairs. The Journal of Organic Chemistry 2000, 65 (22) , 7468-7474. …

Preparation of Singel GC base pair DNA The oligonucleotide duplexes TCCCACTTCCTCTTAT-3’

Oct 12, 1999 · Both base pairs five and six of duplex B show a large roll (≈10°) (Fig. 2 ), sharply bending the helix axis mostly toward the major groove. The bending in duplex B is …