Examining cells for eGFP expression following transduction with Ad5-CMV-eGFP using a fluorescent microscope is a quick method for estimating transduction efficiency. Ad5-CMV-Cre-eGFP expresses the Cre ...
In addition, the inventors inserted an eGFP gene into the first gene position (promoter proximal). The resulting virus is replication-competent and efficiently expresses GFP in infected cells.
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