image: The specific bacterial 16S primer sets efficiently avoids the amplification of plant organelle DNA and is applied to the qPCR quantitative analysis of plant bacterial communities. view more ...

Beyond direct exposure to antibiotics, resistance is also passed between different bacteria through the transfer of small pieces of bacterial DNA called antibiotic resistance genes. There are over ...

"qPCR is a sensitive method that many people in public health are well trained to do, but it requires primer design and validation which is very time consuming," said Yuqing Mao, a doctoral ...

A typical qPCR reaction starts with a master mix containing DNA polymerase, deoxynucleotide triphosphates (dNTPs), primers, a dye or reporter, and template DNA. 4 The reaction runs in a thermal cycler ...

The qPCR assay was also able to detect bacterial cells up to × 10⁻³ cfu/ml bacterial concentration with highly specific E. amylovora detection. Grower surveys and comparative cost-benefit analysis ...

New PCR assays offer hope in the fight against the environmental spread of antimicrobial resistance Date: October 21, 2021 Source: Newcastle University Summary: Researchers have successfully ...

Lappan, R., et al. (2021) Monitoring of diverse enteric pathogens across environmental and host reservoirs with TaqMan array cards and standard qPCR: a methodological comparison study.

This new qPCR master mix can detect as low as one copy of DNA per microliter from bacterial, viral, fungal, and mammalian sources, helping to ensure contamination-free drug products and accurate ...

News Release 6-Mar-2023 Quantifying genetic variations in bacterial cultures the qSanger way ...